In situ visualization of glucocerebrosidase in human skin tissue: zymography versus activity-based probe labeling
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چکیده
منابع مشابه
Visualization of Active Glucocerebrosidase in Rodent Brain with High Spatial Resolution following In Situ Labeling with Fluorescent Activity Based Probes
Gaucher disease is characterized by lysosomal accumulation of glucosylceramide due to deficient activity of lysosomal glucocerebrosidase (GBA). In cells, glucosylceramide is also degraded outside lysosomes by the enzyme glucosylceramidase 2 (GBA2) of which inherited deficiency is associated with ataxias. The interest in GBA and glucosylceramide metabolism in the brain has grown following the no...
متن کاملUltrasensitive in situ visualization of active glucocerebrosidase molecules.
Deficiency of glucocerebrosidase (GBA) underlies Gaucher disease, a common lysosomal storage disorder. Carriership for Gaucher disease has recently been identified as major risk for parkinsonism. Presently, no method exists to visualize active GBA molecules in situ. We here report the design, synthesis and application of two fluorescent activity-based probes allowing highly specific labeling of...
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comparison of catalytic activity of heteropoly compounds in the synthesis of bis(indolyl)alkanes.
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15 صفحه اولIn situ zymography : topographical considerations * Bruce
In situ gelatin zymography is a simple technique providing valuable information about the cellular and tissue localization of gelatinases. Until recently, the use of this technique has been confined to soft, relatively homogeneous tissue. In this report in situ zymography has been utilized to assess the sub-lamellar location of gelatinases in the hard, semi-keratinized epidermal layer and the a...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 2017
ISSN: 0022-2275
DOI: 10.1194/jlr.m079376